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8:31
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jove.com
Video: Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
14.8K Views. State University of New York. This is a detailed protocol for assembling multi-gene plasmids using the Yeast MoClo kit. This method enables the convenient cloning of a variety of multi-gene classmates. It is ideal for generating a big library of related parts.Begin by setting up the Golden Gate reaction mix. Add 20 femtomoles of ...
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